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The procedures involving mice were evaluated by the Val de Loire ethics committee (CEEA VdL, committee number 19) and took place at the INRAE Platform for Experimental Infection PFIE (UE-1277 PFIE, INRAE Centre-Val-de Loire) Valley research, Nouzilly, France, A total of 90 female mice of the CBA/J line (Janvier Labs, Le Genest-Saint-Isle, France) aged 5 weeks at the start of the experiments were used