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Scurvy, the hallmark disease of severe vitamin C deficiency, displays symptoms resulting from loss of collagen that weakens connective tissues: Skin spots caused by bleeding and bruising from broken blood vessels Swelling or bleeding of gums, and eventual loss of teeth Hair loss Delayed healing of skin wounds Fatigue, malaise Iron-deficiency anemia due to decreased absorption of non-heme iron BENEFITS OF VITAMIN D INJECTION: Vitamin D has vital role in the growth and strength of bone and skin

Protein purification and reconstitution HEK293T cells (ATCC, CRL-3216) were transduced stably as described in ref