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buccal cells
trfA gene under tight control of an inducible promoter for copy-number control of CopyControl clones and clones retrofitted with the EZ-Tn5<oriV/KAN-2> Transposon
(1994) Proc
Reliability
Rec J Exonuclease, 250 U @ 10 U/uL GFRNASC buccal cellsMagnesium dependent digestion of single stranded DNA in a 5 to 3 direction Specific: Digests ssDNA in the 5' to 3 direction Heat Inactivated: Heat at 65C for 20 minutes to kill enzyme activity Flexible: Use to remove primers from completed PCR or to degrade single stranded linear DNA in dsDNA and plasmid preps Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5 to 3 direction. Its activity is dependent on Mg2+. Rec J