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Furthermore, an increased number of Tregs in PBMCs from atopic individuals is associated with the suppression of allergic hyperresponsiveness (Th2-mediated) and allogenic reactivity (Th1-mediated) [326]

Cell suspensions were serially diluted and plated on TSA with 0.4% activated charcoal (Sigma) to neutralize residual antibiotics, allowing enumeration of surviving bacteria