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Cancers , 15 (2), 350

Cleland, W

doi: 10.1016/j.cell.2016.06.001

The oocytes were subsequently incubated in a permeabilizing solution (0.5% Triton-X-100 in PBS-PVA) for 30 min at room temperature, washed twice in PBS-PVA, and then blocked with 2% FCS in PBS for 45 min