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The conspicuous blood cortisol awakening response is the net daily increase in blood cortisol within 1 hour of awakening (282)

Gandhi, R., Sekhani, A., Vyas, B., Parmar, H., Makwana, B., Thakore, S., & Shah, P

Leditschke IA

Following centrifugation at 700 g for 15 min with minimal acceleration and deceleration, PBMCs were collected from the interface, washed with HBSS, and resuspended in RPMI medium [43]